Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PAKAF_RS23880 Genome accession   NZ_LR657304
Coordinates   5180223..5181443 (+) Length   406 a.a.
NCBI ID   WP_016253888.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa PAK     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5175223..5186443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAKAF_RS23860 (PAKAF_04795) nadC 5176802..5177650 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PAKAF_RS23870 (PAKAF_05939) pilA 5177838..5178290 (-) 453 WP_024947914.1 pilin Machinery gene
  PAKAF_RS23875 (PAKAF_04797) pilB 5178519..5180219 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PAKAF_RS23880 (PAKAF_04798) pilC 5180223..5181443 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  PAKAF_RS23885 (PAKAF_04799) pilD 5181447..5182319 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PAKAF_RS23890 (PAKAF_04800) coaE 5182316..5182927 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  PAKAF_RS23895 (PAKAF_04801) yacG 5182924..5183124 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PAKAF_RS23900 (PAKAF_04802) - 5183161..5183370 (-) 210 WP_003094660.1 hypothetical protein -
  PAKAF_RS23905 (PAKAF_04803) - 5183476..5184165 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  PAKAF_RS23910 (PAKAF_04804) - 5184162..5184632 (-) 471 WP_003094664.1 hypothetical protein -
  PAKAF_RS23915 (PAKAF_04805) - 5184629..5185054 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  PAKAF_RS23920 (PAKAF_04806) - 5185187..5185816 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  PAKAF_RS23925 - 5185813..5186262 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44549.37 Da        Isoelectric Point: 9.6983

>NTDB_id=1006510 PAKAF_RS23880 WP_016253888.1 5180223..5181443(+) (pilC) [Pseudomonas aeruginosa PAK]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFEGFGAELPAFTQMIVNLSEFMQEWWFFIILAIAIFGFAFK
ELHKRSQKFRDTLDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1006510 PAKAF_RS23880 WP_016253888.1 5180223..5181443(+) (pilC) [Pseudomonas aeruginosa PAK]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAAGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTGAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAGGGCTTCGACAACCCCAACATGCGCAAGCTTGTGGATGA
AATCAAACAGGAAGTTTCCTCAGGTAACAGCCTAGCCAACTCCTTGAGAAAAAAGCCCCAGTATTTTGACGAGCTTTATT
GCAACCTGGTAGATGCAGGGGAACAGTCTGGCGCCTTGGAAAACCTTCTCGATCGGGTGGCAACCTATAAAGAAAAGACG
GAATCACTGAAAGCCAAGATCAAAAAGGCGATGACCTATCCCATTGCCGTCATCATTGTCGCACTGATTGTATCTGCGAT
CCTCCTGATTAAAGTGGTTCCACAATTTCAGTCGGTCTTTGAAGGTTTCGGCGCGGAACTTCCCGCCTTTACCCAGATGA
TTGTCAATCTATCGGAGTTCATGCAGGAGTGGTGGTTCTTCATCATACTGGCGATAGCGATATTTGGCTTTGCATTCAAA
GAATTGCATAAACGCTCACAAAAATTCCGTGACACACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCTGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

76.543

99.754

0.764
  pilC Acinetobacter baumannii D1279779

61.386

99.507

0.611
  pilC Acinetobacter baylyi ADP1

60.837

100

0.608
  pilC Legionella pneumophila strain ERS1305867

55.051

97.537

0.537
  pilG Neisseria gonorrhoeae MS11

46.173

99.754

0.461
  pilG Neisseria meningitidis 44/76-A

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

42.065

97.783

0.411
  pilC Thermus thermophilus HB27

36.908

98.768

0.365

Multiple sequence alignment